Cloning and Expression of Actin Gene of Schistosoma japonicum Chinese Strain

ZHU Jian-Guo^1,2, LIN Jiao-Jiao¹, FENG Xing-Gang¹, WU Xiang-Fu², ZHOU Yuan-Cong², CAI You-Ming^1*
( ¹Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Science, Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, Shanghai 200232, China； ²Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China )

Abstract    A 1 131 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum(Chinese strain) mRNA with primers designed according to published SmAct2 encoding Schistosoma mansoni actin. Sequence analysis indicated that this fragment, with 92% homology to SmAct2, was a complete open reading fragment (ORF) of actin gene of Schistosoma japonicum (Chinese strain). This gene was cloned into the expression vector pET28a(＋) and subsequently expressed in Escerichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 47 kD .Western blotting showed that the recombinant protein had good reactivity with the rabbit serum immunized with Sj worm antigen, indicating that this gene encode actin of Schistosoma japonicum(Chinese strain).
Key words schistosoma japonicum；actin；gene cloning；gene expression

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